Liming improves the stability of soil microbial community structures against the application of digestate made from dairy wastes

Lime is used to reduce soil acidification in agricultural soils. However, its effects on the microbial community are not well understood. Additionally, known be influenced by fertilizers. question remains whether liming influences magnitude of fertilizers' impact communities. Therefore, an incubation experiment was performed understand effect lime application (pH = 6.5 and 5.5 for soils with without lime, respectively) fertilizer (digestate, urea control) structures, stability gene functions. Soils were sampled weekly after fertilizers a month. For analysis, DNA extracted sequenced targeting 16 S rRNA region. abundances i.e rRNA, ammonia oxidizing archaea (AOA), bacteria (AOB), nitrous oxide reductase (nosZ) nitrite (nirS) quantitative PCR conducted. In results, relative abundance Actinobacteria more strongly digestate soils, while Alphaproteobacteria no soil. NL treatments, had significant operational taxonomic units (146) compared (127), indicating that increased community's stability. Liming copy numbers highest values observed plus treatments. Soil pH AOA, nosZ nirS treatments positive AOB but this case These results indicate type should taken into consideration management functional